Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

Mfr.No.

EK1138

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.032	0.086	0.106	0.196	0.432	0.755	1.370	2.094

Description

Human Nesfatin-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human NUCB2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Nucleobindin-2;DNA-binding protein NEFA;Gastric cancer antigen Zg4;Prepronesfatin;Nesfatin-1;NUCB2;NEFA;

Molecular Weight

50196 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V25-L106

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant mouse CCL8

Subcellular Localization

Golgi apparatus . Membrane ; Peripheral membrane protein . Cytoplasm . Secreted . Endoplasmic reticulum . Nucleus envelope . Golgi retention is mediated by its N-terminal region.

Sample Type

Cell culture supernatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	58	278	993	62	286	1045
Standard deviation	2.55	17.51	57.20	3.41	20.59	74.19
CV (%)	4.4%	7.2%	5.6%	5.5%	7.2%	7.1%

Protein Function

Calcium-binding protein. May have a role in calcium homeostasis.

Background

Nesfatin-1 is a naturally occurring protein molecule produced by the brains of mammals. It is responsible for regulating appetite and production of body fat. Nesfatin-1 is a metabolic polypeptide encoded in the N-terminal region of the protein precursor, Nucleobindin2 (NUCB2). Recombinant human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues. Originally identified as a hypothalamic neuropeptide, Nesfatin-1 is also expressed in other areas of the brain, and in pancreatic islets of Langerhans, gastric endocrine cells and adipocytes. Nesfatin-1 plays a role in hunger and energy regulation in an independent manner.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	58	50	49	55	53	3.67	6.9%
Sample 2	278	249	272	250	262	12.93	4.9%
Sample 3	993	1004	914	846	939	64.06	6.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4925
Antigen	Nesfatin-1/NUCB2/Nucleobindin-2
Gene Name	NUCB2
UniProt ID	P80303
Protein Name	Nucleobindin-2
Tissue Specificity	Predominantly expressed in spleen, testis and normal stomach. .
Sequence	Belongs to the nucleobindin family.

Applications	ELISA
Research Area	Calcium Binding Proteins,Calcium Signaling,Chromatin Binding Proteins,DNA/RNA Binding,Epigenetics and Nuclear Signaling,Signal Transduction,Signaling Pathway

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE21000786	EK1138	96 wells/kit	$499		Add to Cart

Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Nesfatin-1/NUCB2/Nucleobindin-2 ELISA Kit PicoKine®

Rate it now..!!