Matrices listed below were spiked with certain level of NEF3 and the recovery rates were calculated by comparing the measured value to the expected amount of NEF3 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
94
EDTA plasma(n=5)
88-103
97
heparin plasma(n=5)
85-103
92
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of NEF3 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
NEF3/Neurofilament 3/NF-M/NFM/Neurofilament, medium polypeptide
Notes
For Research Use Only
UniProt ID
P07197
Antigen
NEF3
Applications
ELISA
Applications Notes
NEF3 ELISA Kit allows for the in vitro quantitative determination of NEF3 concentrations in serum, plasma, tissue homogenates and other biological fluids.