Human Nedd8 - NEDD8 ELISA Kit

Mfr.No.

E5744Hu

Sensitivity

9.87ng/L

Detection Range

20-3500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

NEDD8;NEDD-8;Neddylin;Neural precursor cell expressed developmentally down-regulated protein 8;Ubiquitin-like protein Nedd8

Molecular Weight

9072

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5744Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	761.81	37.71	4.95
Intra-Assay	2	18	864.27	37.47	4.33
Intra-Assay	3	18	1751.9	65.12	3.72

Background

Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.

Protocol

These standard curves of E5744Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2000ng/L	1.898	1.852	1.815
	1.806
1000ng/L	0.93	0.955	0.918
	0.979
500ng/L	0.563	0.537	0.5
	0.51
250ng/L	0.305	0.253	0.217
	0.202
125ng/L	0.081	0.078	0.041
	0.074
0ng/L	0.026	0.036	0
	0.047

Gene ID	4738
UniProt ID	Q15843

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107106	E5744Hu	48T	$320		Add to Cart
BHE12107106	E5744Hu	96T	$458		Add to Cart

Human Nedd8 - NEDD8 ELISA Kit

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