Human Nectin-4/PVRL4 ELISA Kit PicoKine®

Mfr.No.

EK1536

Sensitivity

<10 pg/ml

Detection Range

31.2 pg/ml - 2,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	31.25	62.5	125	250	500	1000	2000
O.D.	0.026	0.141	0.257	0.469	0.82	1.336	1.832	2.182

Description

Human Nectin-4/PVRL4 ELISA Kit PicoKine® (96 Tests). Quantitate Human NECTIN4 in cell culture supernatants, serum, plasma(heparin, EDTA), cell lysates and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Nectin-4;Ig superfamily receptor LNIR;Nectin cell adhesion molecule 4 ;Poliovirus receptor-related protein 4;Processed poliovirus receptor-related protein 4;NECTIN4 ;LNIR, PRR4, PVRL4;

Molecular Weight

55454 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: G32-S349

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Nectin-4

Subcellular Localization

Cell membrane ; Single-pass type I membrane protein . Cell junction, adherens junction . Colocalizes with MLLT4 at cadherin-based adherens junctions (PubMed:11544254).

Sample Type

Cell culture supernatants, Serum, Plasma, Cell lysates, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	75	209	857	70	228	777
Standard deviation	3.67	13.16	44.56	4.13	16.41	41.18
CV (%)	4.9%	7.2%%	5.2%	5.9%	7.2%	5.3%

Protein Function

Seems to be involved in cell adhesion through trans- homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.

Background

Nectin-4, also known as PVRL4, is an adhesion receptor of the immunoglobulin superfamily and functions in cell-cell adhesion. This gene is mapped to chromosome 1q23.3. The encoded protein contains two immunoglobulin-like (Ig-like) C2-type domains and one Ig-like V-type domain. It is involved in cell adhesion through trans-homophilic and -heterophilic interactions. It is a single-pass type I membrane protein. The soluble form is produced by proteolytic cleavage at the cell surface by the metalloproteinase ADAM17/TACE. The secreted form is found in both breast tumor cell lines and breast tumor patients. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder. Alternatively spliced transcript variants have been found but the full-length nature of the variant has not been determined.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	75	82	86	80	80	3.96	4.9%
Sample 2	209	190	207	207	203	7.69	3.7%
Sample 3	857	1006	836	933	908	67.1	7.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	81607
Antigen	Nectin-4/PVRL4
Gene Name	NECTIN4
UniProt ID	Q96NY8
Protein Name	Nectin-4
Tissue Specificity	Predominantly expressed in placenta. Not detected in normal breast epithelium but expressed in breast carcinoma. .

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001116	EK1536	96 wells/kit	$499		Add to Cart

Human Nectin-4/PVRL4 ELISA Kit PicoKine®

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