Human Nebulette - NEBL ELISA Kit

Mfr.No.

E5743Hu

Sensitivity

9.22ng/L

Detection Range

20-4500ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Actin-binding Z-disk protein;bA165O3.1;C10orf113;LASP2;LNEBL;NEBL;Nebulette

Molecular Weight

116453

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5743Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	958.98	58.12	6.06
Intra-Assay	2	18	1498.37	66.12	4.41
Intra-Assay	3	18	373.06	22.05	5.91

Background

Binds to actin and plays an important role in the assembly of the Z-disk. May functionally link sarcomeric actin to the desmin intermediate filaments in the heart muscle sarcomeres (PubMed:27733623). Isoform 2 might play a role in the assembly of focal adhesion (PubMed:15004028).

Protocol

These standard curves of E5743Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.247	2.284	2.239
	2.322
1200ng/L	1.488	1.518	1.473
	1.549
600ng/L	0.731	0.729	0.684
	0.727
300ng/L	0.515	0.531	0.485
	0.547
150ng/L	0.246	0.262	0.217
	0.279
0ng/L	0.041	0.046	0
	0.05

Gene ID	10529
UniProt ID	O76041

Research Area	Signal Transduction; Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107105	E5743Hu	48T	$320		Add to Cart
BHE12107105	E5743Hu	96T	$458		Add to Cart

Human Nebulette - NEBL ELISA Kit

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