Human Nadphoxidase 3 - NOX3 ELISA Kit

Mfr.No.

E5737Hu

Alternative Names

GP91-3;gp91phox homolog 3;Mitogenic oxidase 2;MOX-2;NADPH oxidase 3;NOX 3;NOX3

Reactivity

Human

Detection Range

0.05-20ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

64935

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.026ng/mL

Background

NADPH oxidase which constitutively produces superoxide upon formation of a complex with CYBA/p22phox. Plays a role in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.

Gene ID	50508
UniProt ID	Q9HBY0
Antigen	NOX3

Research Area

Neuroscience

Protocol

These standard curves of E5737Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/mL	2.031	2.049	1.992
	2.068
6ng/mL	1.364	1.357	1.299
	1.349
3ng/mL	0.78	0.792	0.735
	0.804
1.5ng/mL	0.451	0.483	0.426
	0.515
0.75ng/mL	0.273	0.283	0.226
	0.293
0ng/mL	0.049	0.057	0
	0.066

Precision

We measured random samples of E5737Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.1	0.06	5.6
Intra-Assay	2	18	9.7	0.47	4.8
Intra-Assay	3	18	3.9	0.18	4.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107099	E5737Hu	48T	$320		Add to Cart
BHE12107099	E5737Hu	96T	$458		Add to Cart

Human Nadphoxidase 3 - NOX3 ELISA Kit

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