Human Nadphoxidase 1 - NOX1 ELISA Kit

Mfr.No.

E4090hu

Description

This sandwich kit is for the accurate quantitative detection of human NADPH Oxidase 1 (also known as NOX1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Mitogenic oxidase 1;MOX-1;NADH/NADPH mitogenic oxidase subunit P65-MOX;NADPH oxidase 1;NOH-1;NOX 1;NOX1;NOX-1

Reactivity

Human

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

64871

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.09ng/ml

Background

NOH-1S is a voltage-gated proton Channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.

Gene ID	27035
UniProt ID	Q9Y5S8
Antigen	NOX1

Research Area

Others

Protocol

These standard curves of E4090hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.213	2.164	2.117
	2.116
16ng/ml	1.333	1.358	1.311
	1.383
8ng/ml	0.71	0.642	0.595
	0.574
4ng/ml	0.426	0.38	0.333
	0.334
2ng/ml	0.137	0.157	0.11
	0.177
0ng/ml	0.052	0.047	0
	0.042

Precision

We measured random samples of E4090hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	15.5	0.51	3.3
Intra-Assay	2	18	10.9	0.46	4.2
Intra-Assay	3	18	5	0.26	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105497	E4090hu	48T	$320		Add to Cart
BHE12105497	E4090hu	96T	$458		Add to Cart

Human Nadphoxidase 1 - NOX1 ELISA Kit

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