Human Nadp-dependent Malic Enzyme

Mfr.No.

E3967Hu

Description

This sandwich kit is for the accurate quantitative detection of human NADP-dependent Malic Enzyme (also known as ME1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

HUMNDME;Malic enzyme 1;ME1;MES;NADP-dependent malic enzyme;NADP-ME

Reactivity

Human

Detection Range

0.2-60ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

64150

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.107ng/ml

Background

NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene. This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate to pyruvate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Gene ID	4199
UniProt ID	P48163
Antigen	ME1

Research Area

Signal Transduction

Protocol

These standard curves of E3967Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.203	2.268	2.224
	2.334
16ng/ml	1.509	1.551	1.507
	1.593
8ng/ml	1.119	1.145	1.101
	1.172
4ng/ml	0.659	0.663	0.619
	0.667
2ng/ml	0.476	0.483	0.439
	0.49
0ng/ml	0.056	0.044	0
	0.032

Precision

We measured random samples of E3967Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.72	0.35	6.12
Intra-Assay	2	18	12.13	0.57	4.72
Intra-Assay	3	18	4.14	0.35	8.53

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105400	E3967Hu	48T	$320		Add to Cart
BHE12105400	E3967Hu	96T	$458		Add to Cart

Human Nadp-dependent Malic Enzyme - ME1 ELISA Kit

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