Matrices listed below were spiked with certain level of N-ERC/Mesothelin and the recovery rates were calculated by comparing the measured value to the expected amount of N-ERC/Mesothelin in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-104
96
EDTA plasma(n=5)
88-100
96
heparin plasma(n=5)
89-102
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of N-ERC/Mesothelin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
N-ERC/Mesothelin ELISA Kit allows for the in vitro quantitative determination of N-ERC/Mesothelin concentrations in serum, plasma, tissue homogenates and other biological fluids.