Human Myristoylated Alanine-rich C-kinase Substrate

Mfr.No.

E5724Hu

Sensitivity

14.21ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

80K-L;80K-L protein;MACS;MARCKS;Myristoylated alanine-rich C-kinase substrate;PKCSL;PRKCSL;Protein kinase C substrate, 80 kDa protein, light chain

Molecular Weight

31555

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E5724Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	508.64	33.95	6.67
Intra-Assay	2	18	2253.4	79.45	3.53
Intra-Assay	3	18	810.23	45.68	5.64

Background

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

Protocol

These standard curves of E5724Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.302	2.232	2.182
	2.162
2400ng/L	1.744	1.751	1.701
	1.758
1200ng/L	1.246	1.25	1.2
	1.254
600ng/L	0.707	0.711	0.66
	0.715
300ng/L	0.392	0.414	0.364
	0.437
0ng/L	0.036	0.05	0
	0.064

Gene ID	4082
UniProt ID	P29966

Research Area	Signal Transduction; Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107086	E5724Hu	48T	$320		Add to Cart
BHE12107086	E5724Hu	96T	$458		Add to Cart

Human Myristoylated Alanine-rich C-kinase Substrate - MARCKS ELISA Kit

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