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Home / ELISA Kit

Human Myosin-VI (MYO6) ELISA Kit

SKU : BHE13703629

MYO6 encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. Myosin-VI consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. Myosin-VI maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

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Mfr.No.	KTE61414
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Myosin-VI (MYO6) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO6.
Alternative Names	MYO6; RP3-472A9.1; DFNA22; DFNB37; KIAA0389;
Detection Method	Colorimetric
Kit Components	Human Myosin-VI microplate Human Myosin-VI standard Human Myosin-VI detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Myosin-VI (MYO6) ELISA Kit has high sensitivity and excellent specificity for detection of Human MYO6. No significant cross-reactivity or interference between Human MYO6 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MYO6 encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. Myosin-VI consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. Myosin-VI maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Notes	This Human Myosin-VI (MYO6) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO6 in samples. An antibody specific for MYO6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4646

Applications	ELISA
Applications Notes	This Human Myosin-VI (MYO6) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO6 in samples. An antibody specific for MYO6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703629	KTE61414	48 T	$339		Add to Cart
BHE13703629	KTE61414	96 T	$589		Add to Cart
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BHE13703629	KTE61414	96 Tx50	$24569		Add to Cart

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