Human Myosin Light Chain 4

Mfr.No.

E3417Hu

Sensitivity

0.024ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Myosin Light Chain 4 (also known as MYL4) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

ALC1;AMLC;GT1;MYL4;Myosin light chain 1, embryonic muscle/atrial isoform;Myosin light chain 4;Myosin light chain alkali GT-1 isoform;PRO1957

Molecular Weight

21565

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E3417Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.23	0.16	4.86
Intra-Assay	2	18	6.99	0.37	5.28
Intra-Assay	3	18	10.14	0.24	2.36

Background

Atrial Light Chain-1 (ALC-1), also known as Essential Light Chain, Atrial is a protein that in humans is encoded by the MYL4 gene. LC-1 is expressed in fetal cardiac ventricular and fetal skeletal muscle, as well as fetal and adult cardiac atrial tissue. ALC-1 expression is reactivated in human ventricular myocardium in various cardiac muscle diseases, including hypertrophic cardiomyopathy, dilated cardiomyopathy, ischemic cardiomyopathy and congenital heart diseases.

Protocol

These standard curves of E3417Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	1.817	1.904	1.858
	1.991
6ng/ml	0.971	0.934	0.888
	0.898
3ng/ml	0.563	0.54	0.493
	0.517
1.5ng/ml	0.207	0.222	0.176
	0.238
0.75ng/ml	0.083	0.118	0.071
	0.153
0ng/ml	0.034	0.046	0
	0.059

Gene ID	4635
UniProt ID	P12829

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104944	E3417Hu	48T	$320		Add to Cart
BHE12104944	E3417Hu	96T	$458		Add to Cart

Human Myosin Light Chain 4 - MYL4 ELISA Kit

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