Human Myosin Binding Protein C - Cardiac

Mfr.No.

E3757Hu

Sensitivity

0.021ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Myosin Binding Protein C, Cardiac (also known as MYBPC3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Cardiac MyBP-C;C-protein, cardiac muscle isoform;MYBPC 3;MYBPC3;Myosin-binding protein C, cardiac-type

Molecular Weight

140762

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E3757Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	4.5	0.17	3.7
Intra-Assay	2	18	11.5	0.44	3.8
Intra-Assay	3	18	16.9	0.44	2.6

Background

Thick filament-associated protein located in the crossbridge region of vertebrate striated muscle a bands. In vitro it binds MHC, F-actin and native thin filaments, and modifies the activity of actin-activated myosin ATPase. It may modulate muscle contraction or may play a more structural role.

Protocol

These standard curves of E3757Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.057	2.045	1.984
	2.034
10ng/ml	1.078	1.056	0.994
	1.034
5ng/ml	0.446	0.472	0.41
	0.497
2.5ng/ml	0.268	0.241	0.179
	0.214
1.25ng/ml	0.074	0.085	0.023
	0.095
0ng/ml	0.059	0.062	0
	0.065

Gene ID	4607
UniProt ID	Q14896

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105218	E3757Hu	48T	$320		Add to Cart
BHE12105218	E3757Hu	96T	$458		Add to Cart

Human Myosin Binding Protein C - Cardiac - MYBPC3 ELISA Kit

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