Human Myeloperoxidase - MPO ELISA Kit

Mfr.No.

E0880Hu

Description

This sandwich kit is for the accurate quantitative detection of Myeloperoxidase (also known as MPO) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

84 kDa myeloperoxidase;89 kDa myeloperoxidase;MPO;Myeloperoxidase;Myeloperoxidase heavy chain;Myeloperoxidase light chain

Reactivity

Human

Detection Range

0.1-30ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

83869

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.05ng/ml

Background

Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity.

Gene ID	4353
UniProt ID	P05164
Antigen	MPO

Research Area

Immunology

Protocol

These standard curves of E0880Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
16ng/ml	2.157	2.208	2.139
	2.258
8ng/ml	1.373	1.41	1.341
	1.448
4ng/ml	0.671	0.675	0.606
	0.678
2ng/ml	0.505	0.475	0.406
	0.445
1ng/ml	0.253	0.275	0.206
	0.296
0ng/ml	0.062	0.069	0
	0.076

Precision

We measured random samples of E0880Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.8	0.22	5.8
Intra-Assay	2	18	6.3	0.41	6.5
Intra-Assay	3	18	11	0.34	3.1

Storage	2-8°C

Reference	Resveratrol reduces albuminuria in diabetic nephropathy: A randomized double-blind placebo-controlled clinical trial Journal: Diabetes Metab. 2019 Jan;45(1):53-59

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102555	E0880Hu	48T	$320		Add to Cart
BHE12102555	E0880Hu	96T	$458		Add to Cart

Human Myeloperoxidase - MPO ELISA Kit

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