Human MyD88 ELISA Kit PicoKine®

Mfr.No.

EK0942

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.033	0.064	0.159	0.236	0.401	0.705	1.110	1.932

Description

Human MyD88 ELISA Kit PicoKine® (96 Tests). Quantitate Human MYD88 in cell culture supeatants, cell lysates, bone tissue, serum and plasma (heparin, EDTA) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Myeloid differentiation primary response protein MyD88;MYD88;

Molecular Weight

33233 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M1-P296

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human MyD88

Subcellular Localization

Cytoplasm .

Sample Type

Cell culture supeatants, Cell lysates, Bone tissue homogenate, Serum, Plasma

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	244	985	5638	235	1056	5584
Standard deviation	19.27	61.07	332.64	21.15	76.03	429.96
CV (%)	7.9%	6.2%	5.9%	9%	7.2%	7.7%

Protein Function

Adapter protein involved in the Toll-like receptor and IL-1 receptor signaling pathway in the innate immune response. Acts via IRAK1, IRAK2, IRF7 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Increases IL-8 transcription. Involved in IL-18-mediated signaling pathway. Activates IRF1 resulting in its rapid migration into the nucleus to mediate an efficient induction of IFN-beta, NOS2/INOS, and IL12A genes. MyD88-mediated signaling in intestinal epithelial cells is crucial for maintenance of gut homeostasis and controls the expression of the antimicrobial lectin REG3G in the small intestine. .

Background

Myeloid differentiation primary response gene (88) (MYD88) is a protein that, in humans, is encoded by the MYD88 gene. By interspecific backcross mapping, the mouse MyD88 gene was localized to chromosome 9; the human homolog was mapped to 3p22-p21.3 The human ortholog MYD88 seems to function similarly to mice, however, available evidence suggests that MYD88 is dispensable for human resistance to common viral infections and to all but a few pyogenic bacterial infections, demonstrating a major difference between mouse and human immune responses.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	244	207	232	211	223	15.17	6.8%
Sample 2	985	915	1056	940	974	53.57	5.5%
Sample 3	5638	5953	5999	5631	5805	171.54	2.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4615
Gene Name	MYD88
UniProt ID	Q99836
Protein Name	Myeloid differentiation primary response protein MyD88
Tissue Specificity	Ubiquitous. .
Sequence	Contains 1 death domain.

Applications	ELISA
Research Area	Adapters,Atherosclerosis,Cardiovascular,Cytoplasmic,Immunology,Innate Immunity,Signal Transduction,TLR Signaling,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000715	EK0942	96 wells/kit	$499		Add to Cart

Human MyD88 ELISA Kit PicoKine®

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