Human Muscarinic Acetylcholine Receptor M4

Mfr.No.

E3026Hu

Description

This sandwich kit is for the accurate quantitative detection of human Muscarinic Acetylcholine Receptor M4 (also known as M-ACHR M4) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CHRM 4;CHRM4;Muscarinic acetylcholine receptor M4

Reactivity

Human

Detection Range

20-4800ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

53049

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

11.28ng/L

Background

The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium Channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.

Gene ID	1132
UniProt ID	P08173
Antigen	CHRM4

Research Area

Neuroscience

Protocol

These standard curves of E3026Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2560ng/L	2.26	2.183	2.146
	2.107
1280ng/L	1.302	1.331	1.293
	1.359
640ng/L	0.684	0.681	0.644
	0.679
320ng/L	0.381	0.456	0.418
	0.531
160ng/L	0.228	0.221	0.184
	0.214
0ng/L	0.046	0.037	0
	0.029

Precision

We measured random samples of E3026Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1542.2	57.05	3.7
Intra-Assay	2	18	610.2	24.85	4.1
Intra-Assay	3	18	796.7	40.67	5.1

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104563	E3026Hu	48T	$320		Add to Cart
BHE12104563	E3026Hu	96T	$458		Add to Cart

Human Muscarinic Acetylcholine Receptor M4 - M-ACHR M4 ELISA Kit

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