Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine®

Mfr.No.

EK0814

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.000	0.045	0.077	0.148	0.302	0.635	1.173	1.985

Description

Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine® (96 Tests). Quantitate Human MST1 in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Hepatocyte growth factor-like protein;Macrophage stimulatory protein;Macrophage-stimulating protein;MSP;Hepatocyte growth factor-like protein alpha chain;Hepatocyte growth factor-like protein beta chain;MST1;D3F15S2, DNF15S2, HGFL;

Molecular Weight

80320 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-G711

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human MSP

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	250	1105	6318	245	1017	6055
Standard deviation	19.25	44.2	398.03	23.52	53.9	399.63
CV (%)	7.7%	4%	5.3%%	9.6%	5.3%	6.6%

Background

Macrophage Stimulating 1 (SMT1), also known as Hepatocyte growth factor-like protein (HGFL) and MSP, is a protein that in humans is encoded by the MST1 gene. The HGFL gene was identified at the DNF15S2 locus on human chromosome 3 (3p21). The RON tyrosine kinase), the receptor for MSP, is expressed on the ciliated epithelia of the mucociliary transport apparatus of the lung. Furthermore, MSP stimulated ciliary motility in these cells by activating RON.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	250	301	271	291	278	19.56	7%
Sample 2	1105	1177	1061	1161	1126	46.07	4%
Sample 3	6318	6544	5798	6359	6254	277.1	4.4%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4485
Gene Name	MST1
UniProt ID	P26927
Protein Name	Hepatocyte growth factor-like protein
Sequence	Belongs to the peptidase S1 family. Plasminogen subfamily.

Applications	ELISA
Research Area	Cancer,Immunology,Innate Immunity,Macrophage/Inflammation,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000632	EK0814	96 wells/kit	$399		Add to Cart

Human MSP / MST1 / Macrophage Stimulating Protein ELISA Kit PicoKine®

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