Human/Mouse COX-2 ELISA Kit PicoKine®

Mfr.No.

EK2289

Sensitivity

<20 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human, Mouse

Sample Volume

Concentration(pg/ml)	0	156	312	625	1,250	2,500	5,000	10,000
O.D.	0.024	0.069	0.196	0.479	0.657	0.941	1.274	1.814

Description

Human/Mouse COX-2 ELISA Kit PicoKine® (96 Tests). Quantitate Human/Mouse COX-2 in cell lysates. Sensitivity: 20 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Immunogen

Expression system for standard: NS0; Immunogen sequence: A18-L604

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 7

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Sample Type

Cell lysates

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	272	936	2278	187	639	4978
Standard deviation	9.36	41.56	185.01	4.64	45.50	173.73
CV(%)	3.4%	4.4%	6.7%	2.5%	7.1%	3.5%

Background

Cyclooxygenase-2 (COX-2), also known as Prostaglandin-endoperoxide synthase 2 (HUGO PTGS2), is an enzyme that in humans is encoded by the PTGS2 gene. PTGS2 (prostaglandin-endoperoxide synthase 2) is a gene that encodes an enzyme responsible for catalyzing the conversion of arachidonic acid into prostaglandins. Prostaglandins are lipid signaling molecules that play crucial roles in regulating inflammation, pain, and reproductive processes. PTGS2 is expressed in various tissues, particularly in sites of inflammation and certain tumor cells, where its expression levels are often significantly elevated. The activity of this gene is regulated by various stimuli, including cytokines, hormones, and growth factors, making it a key player in pathological conditions such as inflammation, cancer, and cardiovascular diseases. Due to its involvement in these diseases, PTGS2 has become a potential drug target, especially in the development of nonsteroidal anti-inflammatory drugs (NSAIDs).

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	272	267	297	294	283	8.19	2.9%
Sample 2	936	753	1076	1097	966	37.85	3.9%
Sample 3	2778	4231	3055	4660	3681	293.74	8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	5743
Gene Name	PTGS2
UniProt ID	P35354

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001839	EK2289	96 wells/kit	$750		Add to Cart

Human/Mouse COX-2 ELISA Kit PicoKine®

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