Human Mothers Against Decapentaplegic Homolog 7

Mfr.No.

E0639Hu

Description

This sandwich kit is for the accurate quantitative detection of Mothers Against Decapentaplegic Homolog 7 (also known as Smad7) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

hSMAD7;MAD homolog 7;MAD homolog 8;Mothers against decapentaplegic homolog 7;Mothers against decapentaplegic homolog 8;Mothers against DPP homolog 7;Mothers against DPP homolog 8;SMAD 7;SMAD family member 7;SMAD7

Reactivity

Human

Detection Range

0.05-20ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

46426

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.022ng/ml

Background

Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Gene ID	4092
UniProt ID	O15105
Antigen	SMAD7

Research Area

Signal Transduction; Epigenetics and Nuclear Signaling

Protocol

These standard curves of E0639Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.33	2.171	2.108
	2.013
6ng/ml	1.299	1.318	1.255
	1.337
3ng/ml	0.722	0.81	0.747
	0.899
1.5ng/ml	0.549	0.545	0.482
	0.542
0.75ng/ml	0.272	0.286	0.222
	0.299
0ng/ml	0.064	0.063	0
	0.063

Precision

We measured random samples of E0639Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.6	0.24	6.7
Intra-Assay	2	18	7.7	0.25	3.3
Intra-Assay	3	18	1.2	0.07	6.3

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12102321	E0639Hu	96T	$458		Add to Cart
BHE12102321	E0639Hu	48T	$320		Add to Cart

Human Mothers Against Decapentaplegic Homolog 7 - SMAD7 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Mothers Against Decapentaplegic Homolog 7 - SMAD7 ELISA Kit

Rate it now..!!