Human Mothers Against Decapentaplegic Homolog 2

Mfr.No.

E2911Hu

Description

This sandwich kit is for the accurate quantitative detection of human Mothers Against Decapentaplegic Homolog 2 (also known as Smad2) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

hMAD-2;hSMAD2;JV18-1;MAD homolog 2;Mad-related protein 2;Mothers against decapentaplegic homolog 2;Mothers against DPP homolog 2;SMAD 2;SMAD family member 2;SMAD2

Reactivity

Human

Detection Range

0.05-10ng/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

52306

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.019ng/ml

Background

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

Gene ID	4087
UniProt ID	Q15796
Antigen	SMAD2

Research Area

Signal Transduction

Protocol

These standard curves of E2911Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.416	2.331	2.276
	2.247
3.2ng/ml	1.305	1.373	1.318
	1.441
1.6ng/ml	0.868	0.819	0.763
	0.769
0.8ng/ml	0.43	0.46	0.405
	0.49
0.4ng/ml	0.315	0.285	0.23
	0.255
0ng/ml	0.054	0.055	0
	0.056

Precision

We measured random samples of E2911Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1	0.05	5.6
Intra-Assay	2	18	1.9	0.1	5
Intra-Assay	3	18	4.1	0.15	3.6

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104448	E2911Hu	48T	$320		Add to Cart
BHE12104448	E2911Hu	96T	$458		Add to Cart

Human Mothers Against Decapentaplegic Homolog 2 - SMAD2 ELISA Kit

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