Human Monocyte Chemotactic Protein 3

Mfr.No.

E0126Hu

Description

This sandwich kit is for the accurate quantitative detection of human Monocyte Chemotactic Protein 3 (also known as MCP-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

C-C motif chemokine 7;CCL 7;CCL7;MCP-3;Monocyte chemoattractant protein 3;Monocyte chemotactic protein 3;NC28;Small-inducible cytokine A7

Reactivity

Human

Detection Range

5-1000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

11200

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

3.07ng/L

Background

Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3.

Gene ID	6354
UniProt ID	P80098
Antigen	CCL7

Research Area

Immunology

Protocol

These standard curves of E0126Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/L	2.2	2.121	2.074
	2.041
320ng/L	1.784	1.832	1.785
	1.88
160ng/L	1.268	1.223	1.176
	1.177
80ng/L	0.7	0.674	0.627
	0.647
40ng/L	0.434	0.431	0.384
	0.427
0ng/L	0.042	0.047	0
	0.052

Precision

We measured random samples of E0126Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	41.7	1.9	4.6
Intra-Assay	2	18	343.1	21.63	6.3
Intra-Assay	3	18	107.6	5.03	4.7

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101861	E0126Hu	96T	$458		Add to Cart
BHE12101861	E0126Hu	48T	$320		Add to Cart

Human Monocyte Chemotactic Protein 3 - MCP-3 ELISA Kit

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