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Home / ELISA Kit

Human Mitogen-activated protein kinase 14 (MAPK14) ELISA Kit

SKU : BHE13703948

Mitogen-activated protein kinase 14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

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Mfr.No.	KTE61733
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Mitogen-activated protein kinase 14 (MAPK14) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK14.
Alternative Names	MAPK14; RP1-179N16.5; CSBP1; CSBP2; CSPB1; EXIP; Mxi2; PRKM14; PRKM15; RK; SAPK2A; p38; p38ALPHA; Csaids binding protein; MAP kinase Mxi2; MAX-interacting protein 2; cytokine suppressive anti-inflammatory drug
Detection Method	Colorimetric
Kit Components	Human Mitogen-activated protein kinase 14 microplate Human Mitogen-activated protein kinase 14 standard Human Mitogen-activated protein kinase 14 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Mitogen-activated protein kinase 14 (MAPK14) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAPK14. No significant cross-reactivity or interference between Human MAPK14 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Mitogen-activated protein kinase 14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.
Notes	This Human Mitogen-activated protein kinase 14 (MAPK14) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK14 in samples. An antibody specific for MAPK14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK14 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	1432

Applications	ELISA
Applications Notes	This Human Mitogen-activated protein kinase 14 (MAPK14) ELISA Kit employs a two-site sandwich ELISA to quantitate MAPK14 in samples. An antibody specific for MAPK14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAPK14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAPK14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAPK14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703948	KTE61733	48 T	$339		Add to Cart
BHE13703948	KTE61733	96 T	$589		Add to Cart
BHE13703948	KTE61733	96 Tx5	$2629		Add to Cart
BHE13703948	KTE61733	96 Tx50	$24569		Add to Cart

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