Human Mitogen Activated Protein Kinase 1

Mfr.No.

E2492Hu

Alternative Names

MAP3K1;MAPK/ERK kinase 1;MEK kinase 1;MEKK 1;Mitogen-activated protein kinase kinase 1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

164470

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.55ng/L

Background

Component of a protein kinase signal transduction cascade (PubMed: 9808624). Activates the ERK and JNK kinase pathways by phosphorylation of MAP2K1 and MAP2K4 (PubMed: 9808624). May phosphorylate the MAPK8/JNK1 kinase (PubMed: 17761173). Activates CHUK and IKBKB, the central protein kinases of the NF-kappa-B pathway (PubMed: 9808624).

Gene ID	4214
UniProt ID	Q13233
Antigen	MAP3K1

Research Area

Signal Transduction

Protocol

These standard curves of E2492Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.417	2.374	2.339
	2.332
1200ng/L	1.396	1.305	1.269
	1.213
600ng/L	0.728	0.727	0.691
	0.725
300ng/L	0.316	0.336	0.3
	0.356
150ng/L	0.165	0.188	0.153
	0.211
0ng/L	0.049	0.036	0
	0.022

Precision

We measured random samples of E2492Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	749.3	34.31	4.6
Intra-Assay	2	18	1713.5	46.18	2.7
Intra-Assay	3	18	351.2	15.54	4.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12104030	E2492Hu	48T	$320		Add to Cart
BHE12104030	E2492Hu	96T	$458		Add to Cart

Human Mitogen Activated Protein Kinase 1 - MAP3K1 ELISA Kit

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