Human Mitochondrial Uncoupling Protein 2

Mfr.No.

E5683Hu

Sensitivity

13.45ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Mitochondrial uncoupling protein 2;Solute carrier family 25 member 8;UCP 2;UCP2;UCPH

Molecular Weight

33229

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5683Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3948.4	141.39	3.6
Intra-Assay	2	18	2513	120.55	4.8
Intra-Assay	3	18	1358.8	71.89	5.3

Background

UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.

Protocol

These standard curves of E5683Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.06	1.981	1.935
	1.903
2400ng/L	0.928	1.036	0.99
	1.144
1200ng/L	0.455	0.519	0.473
	0.583
600ng/L	0.236	0.198	0.152
	0.161
300ng/L	0.146	0.118	0.072
	0.091
0ng/L	0.062	0.046	0
	0.03

Gene ID	7351
UniProt ID	P55851

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107045	E5683Hu	48T	$320		Add to Cart
BHE12107045	E5683Hu	96T	$458		Add to Cart

Human Mitochondrial Uncoupling Protein 2 - UCP2 ELISA Kit

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