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Home / ELISA Kit

Human Midkine (MK) ELISA Kit

SKU : BHE13703893

MK encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. Midkine promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. MK has been targeted as a therapeutic for a variety of different disorders. Alternatively spliced transcript variants encoding multiple isoforms have been observed.

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Abbkine Scientific Co., Ltd.

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Mfr.No.	KTE61678
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Midkine (MK) ELISA Kit employs a two-site sandwich ELISA to quantitate MDK.
Alternative Names	MDK; FLJ27379; MK; NEGF2; OTTHUMP00000197041; OTTHUMP00000197042; OTTHUMP00000197045; midkine
Detection Method	Colorimetric
Kit Components	Human Midkine microplate Human Midkine standard Human Midkine detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Midkine (MK) ELISA Kit has high sensitivity and excellent specificity for detection of Human MDK. No significant cross-reactivity or interference between Human MDK and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MK encodes a member of a small family of secreted growth factors that binds heparin and responds to retinoic acid. Midkine promotes cell growth, migration, and angiogenesis, in particular during tumorigenesis. MK has been targeted as a therapeutic for a variety of different disorders. Alteatively spliced transcript variants encoding multiple isoforms have been observed.
Notes	This Human Midkine (MK) ELISA Kit employs a two-site sandwich ELISA to quantitate MDK in samples. An antibody specific for MDK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4192

Applications	ELISA
Applications Notes	This Human Midkine (MK) ELISA Kit employs a two-site sandwich ELISA to quantitate MDK in samples. An antibody specific for MDK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMDK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MDK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MDK bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703893	KTE61678	48 T	$359		Add to Cart
BHE13703893	KTE61678	96 T	$619		Add to Cart
BHE13703893	KTE61678	96 Tx5	$2809		Add to Cart
BHE13703893	KTE61678	96 Tx50	$26209		Add to Cart

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