Human Microtubule-associated Proteins 1A - 1B Light Chain 3A

Mfr.No.

E6855Hu

Alternative Names

Autophagy-related protein LC3 A;Autophagy-related ubiquitin-like modifier LC3 A;MAP1 light chain 3-like protein 1;MAP1A/MAP1B LC3 A;MAP1A/MAP1B light chain 3 A;MAP1LC3A;Microtubule-associated protein 1 light chain 3 alpha;Microtubule-associated proteins 1A/1B light chain 3A

Reactivity

Human

Detection Range

20-6000ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

14272

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

11.63ng/L

Background

Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes) (PubMed: 20713600, PubMed: 24290141). While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation (PubMed: 20713600). Through its interaction with the reticulophagy receptor TEX264, participates in the remodeling of subdomains of the endoplasmic reticulum into autophagosomes upon nutrient stress, which then fuse with lysosomes for endoplasmic reticulum turnover (PubMed: 31006538, PubMed: 31006537).

Gene ID	84557
UniProt ID	Q9H492
Antigen	MAP1LC3A

Research Area

Signal Transduction

Protocol

These standard curves of E6855Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
3200ng/L	2.208	2.195	2.15
	2.183
1600ng/L	1.832	1.794	1.748
	1.756
800ng/L	1.251	1.182	1.137
	1.114
400ng/L	0.663	0.694	0.648
	0.725
200ng/L	0.429	0.435	0.389
	0.441
0ng/L	0.044	0.046	0
	0.047

Precision

We measured random samples of E6855Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1399.3	73.36	5.2
Intra-Assay	2	18	488.4	26.59	5.4
Intra-Assay	3	18	175.5	10.74	6.1

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12115561	E6855Hu	48T	$320		Add to Cart
BHE12115561	E6855Hu	96T	$458		Add to Cart

Human Microtubule-associated Proteins 1A - 1B Light Chain 3A - MAPILC3A ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Human Microtubule-associated Proteins 1A - 1B Light Chain 3A - MAPILC3A ELISA Kit

Rate it now..!!