Human Microtubule-associated Protein 1B

Mfr.No.

E4782Hu

Sensitivity

7.58ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

MAP1 light chain LC1;MAP1B;MAP-1B;MAP1B heavy chain;Microtubule-associated protein 1B

Molecular Weight

270634

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E4782Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	388.2	18.02	4.6
Intra-Assay	2	18	1102.6	39.46	3.6
Intra-Assay	3	18	476.4	19.25	4

Background

Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.

Protocol

These standard curves of E4782Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.122	2.162	2.129
	2.201
800ng/L	1.41	1.384	1.351
	1.357
400ng/L	0.653	0.642	0.609
	0.63
200ng/L	0.39	0.354	0.321
	0.317
100ng/L	0.201	0.185	0.153
	0.17
0ng/L	0.023	0.032	0
	0.042

Gene ID	4131
UniProt ID	P46821

Research Area	Neuroscience

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105542	E4782Hu	48T	$320		Add to Cart
BHE12105542	E4782Hu	96T	$458		Add to Cart

Human Microtubule-associated Protein 1B - MAP1B ELISA Kit

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