Human MIA ELISA Kit PicoKine®

Mfr.No.

EK0941

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.021	0.054	0.089	0.156	0.352	0.892	1.396	2.046

Description

Human MIA ELISA Kit PicoKine® (96 Tests). Quantitate Human MIA in cell culture supeatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Melanoma-derived growth regulatory protein;Melanoma inhibitory activity protein;MIA;

Molecular Weight

14509 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G25-131Q

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human MIA

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	29	172	485	26	165	509
Standard deviation	1.91	13.41	32.98	1.97	15.84	36.92
CV (%)	6.6%	7.8%	6.8%	7.6%	9.6%	6.9%

Protein Function

Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.

Background

Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	29	28	27	29	28	0.82	2.9%
Sample 2	172	166	171	170	169	2.27	1.3%
Sample 3	485	453	511	458	476	23.22	4.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	8190
Gene Name	MIA
UniProt ID	Q16674
Protein Name	Melanoma-derived growth regulatory protein
Tissue Specificity	All malignant melanoma cell lines tested and infrequently in glioma cell lines.
Sequence	Belongs to the MIA/OTOR family.

Applications	ELISA
Research Area	Cancer,Cytokines,Growth Factors/Hormones,Immunology,Innate Immunity,Secreted Molecules,Signal Transduction,Tumor Immunology

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000714	EK0941	96 wells/kit	$499		Add to Cart

Human MIA ELISA Kit PicoKine®

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