Human MHC class I polypeptide-related sequence B(MICB) ELISA kit

Human MHC class I polypeptide-related sequence B(MICB) ELISA kit

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Mfr.No.

CSB-EL013813HU

Sensitivity

7.8 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

MHC class I polypeptide-related sequence B, DAAP-210H10.1, PERB11.2, MHC class I chain-related protein B, MHC class I mic-B antigen, MHC class I molecule, MHC class I-like molecule PERB11.2-IMX, stress inducible class I homolog

Recovery

The recovery of human IMA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	96	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human IMA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-90
1:2	Average %	99
	Range %	95-102
1:4	Average %	103
	Range %	98-107
1:8	Average %	94
	Range %	88-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

IU/ml	OD1	OD2	Average	Corrected
200	2.497	2.467	2.482	2.363
100	2.013	1.973	1.993	1.874
50	1.300	1.288	1.294	1.175
25	0.687	0.705	0.696	0.577
12.5	0.442	0.453	0.448	0.329
6.25	0.283	0.306	0.295	0.176
3.12	0.198	0.210	0.204	0.085
0	0.121	0.117	0.119	?

Species

Homo sapiens

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q29980
Tissue Specificity	Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in s

Applications	ELISA
Research Area	MICB

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10503349	CSB-EL013813HU	96T	$695	Add to Cart
BHE10503349	CSB-EL013813HU	5x96T	$3336	Add to Cart
BHE10503349	CSB-EL013813HU	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.