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Home / ELISA Kit

Human Methylthioribose-1-phosphate isomerase (MRI1) ELISA Kit

SKU : BHE13703753

MRI1 (Methylthioribose-1-Phosphate Isomerase 1) is a Protein Coding gene. Diseases associated with MRI1 include Spinal Cord Melanoma and Visual Agnosia. Among its related pathways are Metabolism and Sulfur amino acid metabolism. GO annotations related to this gene include identical protein binding and S-methyl-5-thioribose-1-phosphate isomerase activity. This enzyme functions in the methionine salvage pathway by catalyzing the interconversion of methylthioribose-1-phosphate and methythioribulose-1-phosphate. Elevated expression of the encoded protein is associated with metastatic melanoma and this protein promotes melanoma cell invasion independent of its enzymatic activity. Mutations in this gene may be associated with vanishing white matter disease (VMWD).

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Mfr.No.	KTE61538
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Methylthioribose-1-phosphate isomerase (MRI1) ELISA Kit employs a two-site sandwich ELISA to quantitate MRI1.
Alternative Names	MRI1; MGC3207; MTNA; Ypr118w; translation initiation factor eIF-2B subunit alpha/beta/delta-like protein
Detection Method	Colorimetric
Kit Components	Human Methylthioribose-1-phosphate isomerase microplate Human Methylthioribose-1-phosphate isomerase standard Human Methylthioribose-1-phosphate isomerase detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Methylthioribose-1-phosphate isomerase (MRI1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MRI1. No significant cross-reactivity or interference between Human MRI1 and analogues was observed.
Calibration Range	Please inquire
Sample Type	Serum, Cell culture supeatants, Plasma, Other biological fluids
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	MRI1 (Methylthioribose-1-Phosphate Isomerase 1) is a Protein Coding gene. Diseases associated with MRI1 include Spinal Cord Melanoma and Visual Agnosia. Among its related pathways are Metabolism and Sulfur amino acid metabolism. GO annotations related to this gene include identical protein binding and S-methyl-5-thioribose-1-phosphate isomerase activity. This enzyme functions in the methionine salvage pathway by catalyzing the interconversion of methylthioribose-1-phosphate and methythioribulose-1-phosphate. Elevated expression of the encoded protein is associated with metastatic melanoma and this protein promotes melanoma cell invasion independent of its enzymatic activity. Mutations in this gene may be associated with vanishing white matter disease (VMWD).
Notes	This Human Methylthioribose-1-phosphate isomerase (MRI1) ELISA Kit employs a two-site sandwich ELISA to quantitate MRI1 in samples. An antibody specific for MRI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	534734

Applications	ELISA
Applications Notes	This Human Methylthioribose-1-phosphate isomerase (MRI1) ELISA Kit employs a two-site sandwich ELISA to quantitate MRI1 in samples. An antibody specific for MRI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMRI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MRI1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MRI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703753	KTE61538	48 T	$339		Add to Cart
BHE13703753	KTE61538	96 T	$589		Add to Cart
BHE13703753	KTE61538	96 Tx5	$2629		Add to Cart
BHE13703753	KTE61538	96 Tx50	$24569		Add to Cart

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