Human Metastasis-associated in colon cancer protein 1(MACC1) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EL013298HU

Sensitivity

11.75 pg/mL

Detection Range

47 pg/mL-3000 pg/mL

Reactivity

Human

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

metastasis associated in colon cancer 1, 7A5, SH3BP4L, OTTHUMP00000201891, putative binding protein 7a5

Recovery

The recovery of human KLK 1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	92-101
EDTA plasma (n=4)	89	85-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human KLK 1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	85-103
1:2	Average %	90
	Range %	85-99
1:4	Average %	93
	Range %	85-97
1:8	Average %	92
	Range %	83-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Homo sapiens

Sample Type

Cell lysates, Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q6ZN28
Tissue Specificity	Preferentially expressed in metastasizing tumors.

Applications	ELISA
Research Area	MACC1

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10503338	CSB-EL013298HU	96T	$695	Add to Cart
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