Human Mesothelin - MSLN ELISA Kit

Mfr.No.

E2191Hu

Sensitivity

0.01ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Mesothelin (also known as MSLN) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CAK1 antigen;Megakaryocyte-potentiating factor;Mesothelin;Mesothelin, cleaved form;MPF;MSLN;Pre-pro-megakaryocyte-potentiating factor

Molecular Weight

68986

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E2191Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.1	0.17	2.8
Intra-Assay	2	18	4.5	0.19	4.3
Intra-Assay	3	18	1	0.05	5.4

Background

Membrane-anchored forms may play a role in cellular adhesion. Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.

Protocol

These standard curves of E2191Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.137	2.217	2.171
	2.296
6ng/ml	1.393	1.412	1.367
	1.432
3ng/ml	0.813	0.864	0.819
	0.914
1.5ng/ml	0.551	0.501	0.456
	0.452
0.75ng/ml	0.245	0.276	0.231
	0.306
0ng/ml	0.046	0.045	0
	0.044

Gene ID	10232
UniProt ID	Q13421

Research Area	Cancer

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103732	E2191Hu	48T	$320		Add to Cart
BHE12103732	E2191Hu	96T	$458		Add to Cart

Human Mesothelin - MSLN ELISA Kit

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