Human Mesothelin ELISA Kit PicoKine®

Mfr.No.

EK1338

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	125000	25000	5000	10,000
O.D.	0.041	0.125	0.190	0.282	0.495	0.815	1.237	1.772

Description

Human Mesothelin ELISA Kit PicoKine® (96 Tests). Quantitate Human MSLN in cell culture supeatants, serum, plasma (heparin, EDTA) , saliva and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Mesothelin;CAK1 antigen;Pre-pro-megakaryocyte-potentiating factor;Megakaryocyte-potentiating factor;MPF;Mesothelin, cleaved form;MSLN;MPF;

Molecular Weight

68986 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: E296-G580

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Mesothelin

Subcellular Localization

Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.

Sample Type

Cell culture supeatants, Serum, Plasma, Saliva, Urine

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	307	1077	6075	320	1138	6301
Standard deviation	12.58	52.77	443.47	17.28	70.55	516.68
CV (%)	4.1%	4.9%	7.3%	5.4%	6.2%	8.2%

Protein Function

Membrane-anchored forms may play a role in cellular adhesion.

Background

Mesothelin, also known as MSLN or MPF is a protein that in humans is encoded by the MSLN gene. This gene is mapped to 16p13.3. This gene encodes a precursor protein that is cleaved into two products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiation factor functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. Mesothelian is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	307	282	307	344	310	22.12	7.1%
Sample 2	1077	1082	1223	1218	1150	70.54	6.1%
Sample 3	6075	5852	5500	5776	5800	205.48	3.5%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	10232
Gene Name	MSLN
UniProt ID	Q13421
Protein Name	Mesothelin
Tissue Specificity	Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level). .

Applications	ELISA
Research Area	Cancer,Cell Type Markers,Tags & Cell Markers,Tumor Antigens,Tumor Associated,Tumor Biomarkers,Tumor Immunology,Tumor-Associated Antigens

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000932	EK1338	96 wells/kit	$499		Add to Cart

Human Mesothelin ELISA Kit PicoKine®

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