Human Melanoma-associated Antigen 1

Mfr.No.

E1698Hu

Description

This sandwich kit is for the accurate quantitative detection of human Melanoma-associated Antigen 1 (also known as MAGEA1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Antigen MZ2-E;Cancer/testis antigen 1.1;CT1.1;MAGE-1 antigen;MAGEA 1;MAGEA1;Melanoma-associated antigen 1

Reactivity

Human

Detection Range

20-4500ng/L

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

34342

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

9.17ng/L

Background

May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

Gene ID	4100
UniProt ID	P43355
Antigen	MAGEA1

Research Area

Cancer

Protocol

These standard curves of E1698Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.27	2.243	2.228
	2.215
1200ng/L	1.342	1.372	1.357
	1.402
600ng/L	0.679	0.665	0.65
	0.651
300ng/L	0.435	0.411	0.397
	0.388
150ng/L	0.208	0.206	0.191
	0.204
0ng/L	0.015	0.015	0
	0.014

Precision

We measured random samples of E1698Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	716.8	44.18	6.2
Intra-Assay	2	18	1321.4	42.8	3.2
Intra-Assay	3	18	504.4	26.14	5.2

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12103280	E1698Hu	48T	$320		Add to Cart
BHE12103280	E1698Hu	96T	$458		Add to Cart

Human Melanoma-associated Antigen 1 - MAGEA1 ELISA Kit

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