Human Melanocyte Protein Pmel

Mfr.No.

E5652Hu

Alternative Names

95 kDa melanocyte-specific secreted glycoprotein;D12S53E;gp100;M-alpha;M-beta);ME20;ME20M;ME20-M;ME20S;ME20-S;Melanocyte protein PMEL;Melanocyte protein Pmel 17;Melanocytes lineage-specific antigen GP100;Melanoma-associated ME20 antigen;P1;P100;P26;PMEL17;Premelanosome protein;Secreted melanoma-associated ME20 antigen;SI;SIL;SILV;Silver locus protein homolog

Reactivity

Human

Detection Range

15-3000ng/L

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

70255

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

7.41ng/L

Background

Plays a central role in the biogenesis of melanosomes. Involved in the maturation of melanosomes from stage I to II. The transition from stage I melanosomes to stage II melanosomes involves an elongation of the vesicle, and the appearance within of distinct fibrillar structures. Release of the soluble form, ME20-S, could protect tumor cells from antibody mediated immunity.

Gene ID	6490
UniProt ID	P40967
Antigen	SILV

Research Area

Others

Protocol

These standard curves of E5652Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.331	2.331	2.295
	2.331
800ng/L	1.767	1.793	1.757
	1.82
400ng/L	1.178	1.136	1.099
	1.093
200ng/L	0.726	0.699	0.663
	0.673
100ng/L	0.397	0.377	0.34
	0.356
0ng/L	0.04	0.036	0
	0.032

Precision

We measured random samples of E5652Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	535.07	22.71	4.24
Intra-Assay	2	18	438.01	29.66	6.77
Intra-Assay	3	18	175.38	11.19	6.38

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12107014	E5652Hu	48T	$320		Add to Cart
BHE12107014	E5652Hu	96T	$458		Add to Cart

Human Melanocyte Protein Pmel - SILV ELISA Kit

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