Human MCP-1 / CCL2 ELISA Kit PicoKine®

Mfr.No.

EK0441

Sensitivity

<1 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.025	0.071	0.154	0.244	0.466	1.052	1.298	1.892

Description

Human MCP-1 / CCL2 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCL2 in cell culture supeatants, serum, plasma (heparin, EDTA, citrate) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 2;HC11;Monocyte chemoattractant protein 1;Monocyte chemotactic and activating factor;MCAF;Monocyte chemotactic protein 1;MCP-1;Monocyte secretory protein JE;Small-inducible cytokine A2;CCL2;MCP1, SCYA2;

Molecular Weight

11025 MW

Immunogen

Expression system for standard: E.coli; Immunogen sequence: Q24-T99

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is cross-reactivity with human Eotaxin, MCP-3<1%.

Specificity

Natural and recombinant human MCP-1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma, Urine

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	31	159	477	30	148	481
Standard deviation	1.55	8.74	32.91	1.98	9.76	37.51
CV (%)	5%	5.5%	6.9%	6.6%	6.6%	7.8%

Protein Function

Chemotactic factor that attracts monocytes and basophils but not neutrophils or eosinophils. Augments monocyte anti-tumor activity. Has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.

Background

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids. MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues. MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder. Human MCP-1 is a 8.7KDa non-glycoprotein, consisting of 99 amino acids in precursor form and 76 amino acids in mature form.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	31	33	37	35	34	2.23	6.5%
Sample 2	159	139	156	158	153	8.15	5.3%
Sample 3	477	421	423	456	444	23.46	5.2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	6347
Gene Name	CCL2
UniProt ID	P13500
Protein Name	C-C motif chemokine 2
Sequence	Belongs to the intercrine beta (chemokine CC) family.

Applications	ELISA
Research Area	Angiogenesis,Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Chemokines,Heart Disease,Immunology,Innate Immunity,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000437	EK0441	96 wells/kit	$399		Add to Cart

Human MCP-1 / CCL2 ELISA Kit PicoKine®

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