Human Matrix Metalloproteinase 1

Mfr.No.

E0916Hu

Description

This sandwich kit is for the accurate quantitative detection of human Matrix Metalloproteinase 1 (also known as MMP-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

22 kDa interstitial collagenase;27 kDa interstitial collagenase;Fibroblast collagenase;Interstitial collagenase;Matrix Metalloproteinase-1;MMP 1;MMP1;MMP-1

Reactivity

Human

Detection Range

0.05-70ng/ml

Sample Type

Serum, Cell culture supernatants, Plasma

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

54007

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.01ng/ml

Background

Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X (PubMed: 2557822, PubMed: 2153297, PubMed: 1645757). In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity (PubMed: 16807369).

Gene ID	4312
UniProt ID	P03956
Antigen	MMP1

Research Area

Cardiovascular; Signal Transduction; Cancer; Cell Biology

Protocol

These standard curves of E0916Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.183	2.111	2.083
	2.04
20ng/ml	1.188	1.219	1.19
	1.249
10ng/ml	0.625	0.709	0.68
	0.793
5ng/ml	0.499	0.503	0.475
	0.507
2.5ng/ml	0.225	0.217	0.188
	0.209
0ng/ml	0.029	0.028	0
	0.028

Precision

We measured random samples of E0916Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	11.3	0.73	6.5
Intra-Assay	2	18	25.2	0.67	2.6
Intra-Assay	3	18	6	0.44	7.3

Storage	2-8°C

Reference	Pulmonary Functions and Inflammatory Biomarkers in Post-Pulmonary Tuberculosis Sequelae Journal: Tuberc Respir Dis (Seoul). 2022 Apr;85(2):175-184 The level of ratio between matrix metalloproteinase-1 (MMP-1) and tissue inhibitor matrix metalloproteinase-1 (TIMP-1) after prolotherapy intervention and the functional outcome in patient with frozen shoulder: A randomized controlled trial Journal: Medicine (Baltimore). 2023 Jul 28;102(30):e34356.

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SKU	Supplier No.	Size	Your price	Quantity
BHE12102591	E0916Hu	48T	$320		Add to Cart
BHE12102591	E0916Hu	96T	$458		Add to Cart

Human Matrix Metalloproteinase 1 - MMP-1 ELISA Kit

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