Human Matrilin-3 ELISA Kit PicoKine®

Mfr.No.

EK2022

Sensitivity

<50 pg/ml

Detection Range

93.7 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.7	187.5	375	750	1500	3000	6000
O.D.	0.110	0.183	0.230	0.386	0.596	0.991	1.661	2.295

Description

Human Matrilin-3 ELISA Kit PicoKine® (96 Tests). Quantitate Human MATN3 in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Matrilin-3; MATN3

Immunogen

Expression system for standard: NS0; Immunogen sequence: D29-R486

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Matrilin-3

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	125	689	2209	148	697	2553
Standard deviation	6.13	29.63	90.57	9.77	40.43	194.03
CV (%)	4.9%	4.3%	4.1%	6.6%	5.8%	7.6%

Protein Function

Major component of the extracellular matrix of cartilage and may play a role in the formation of extracellular filamentous networks.

Background

Matrilin-3 is a protein that in humans is encoded by the MATN3 gene. This gene is mapped to 2p24.1. This gene encodes a member of von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues. This protein contains two von Willebrand factor A domains; it is present in the cartilage extracellular matrix and has a role in the development and homeostasis of cartilage and bone. Mutations in this gene result in multiple epiphyseal dysplasia.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	125	143	138	129	133	7.11	5.3%
Sample 2	689	800	745	802	759	46.43	6.1%
Sample 3	2209	2281	2219	2542	2312	135.20	5.8%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	4148
Gene Name	MATN3
UniProt ID	O15232
Protein Name	Matrilin-3
Tissue Specificity	Expressed only in cartilaginous tissues, such as vertebrae, ribs and shoulders.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001520	EK2022	96 wells/kit	$499		Add to Cart

Human Matrilin-3 ELISA Kit PicoKine®

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