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Home / ELISA Kit

Human Matrilin-2 (MATN2) ELISA Kit

SKU : BHE13703926

Matrilin-2 is a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues.The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.MATN2 contains 2 VWFA-like domains, 10 EGF-type modules, and an oligomerization module. RT-PCR demonstrated wide expression of MATN2 in human cell lines of fibroblastic or epithelial origin. Two mRNA transcripts were detected which differ from each other in a short region that is unique to matrilin-2; 19 triplets are included or spliced out of the mature mRNA as a result of alternative 3-prime splice site selection.

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Mfr.No.	KTE61711
Reactivity	Human
Assay Duration	Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Assay Type	Sandwich (quantitative)
Description	This Human Matrilin-2 (MATN2) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN2.
Alternative Names	MATN2
Detection Method	Colorimetric
Kit Components	Human Matrilin-2 microplate Human Matrilin-2 standard Human Matrilin-2 detect antibody Streptavidin-HRP Standard diluent Assay buffer HRP substrate Stop solution Wash buffer Plate covers
Features	Human Matrilin-2 (MATN2) ELISA Kit has high sensitivity and excellent specificity for detection of Human MATN2. No significant cross-reactivity or interference between Human MATN2 and analogues was observed.
Calibration Range	Please inquire
Limit Of Detection	Please inquire
Precautions	The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background	Matrilin-2 is a member of the von Willebrand factor A domain containing protein family. This family of proteins is thought to be involved in the formation of filamentous networks in the extracellular matrices of various tissues.The specific function of this gene has not yet been determined. Two transcript variants encoding different isoforms have been found for this gene.MATN2 contains 2 VWFA-like domains, 10 EGF-type modules, and an oligomerization module. RT-PCR demonstrated wide expression of MATN2 in human cell lines of fibroblastic or epithelial origin. Two mRNA transcripts were detected which differ from each other in a short region that is unique to matrilin-2; 19 triplets are included or spliced out of the mature mRNA as a result of alternative 3-prime splice site selection.
Notes	This Human Matrilin-2 (MATN2) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN2 in samples. An antibody specific for MATN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMATN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Usage Notes	Do not mix components from different kit lots or use reagents beyond the kit expiration date. Allow all reagents to warm to room temperature for at least 30 minutes before opening. Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination. Unused wells must be kept desiccated at 4 °C in the sealed bag provided. Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes. It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gene ID	4147

Applications	ELISA
Applications Notes	This Human Matrilin-2 (MATN2) ELISA Kit employs a two-site sandwich ELISA to quantitate MATN2 in samples. An antibody specific for MATN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMATN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MATN2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MATN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Storage Instructions	The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.
Shipping	Gel pack with blue ice.

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SKU	Supplier No.	Size	Your price	Quantity
BHE13703926	KTE61711	48 T	$339		Add to Cart
BHE13703926	KTE61711	96 T	$589		Add to Cart
BHE13703926	KTE61711	96 Tx5	$2629		Add to Cart
BHE13703926	KTE61711	96 Tx50	$24569		Add to Cart

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