Human MAP4K4(Mitogen-activated Protein Kinase 4) ELISA Kit

Mfr.No.

ELK0209

Sensitivity

12.4 pg/mL

Detection Range

46.88-3000 pg/mL

Assay Duration

3.5h

Assay Type

Sandwich

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAP4K4 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	98-103%	86-92%	87-93%	93-102%
EDTA Plasma(n=5)	85-92%	79-96%	89-101%	85-97%
Heparin Plasma(n=5)	86-94%	93-102%	89-97%	85-94%

Standard Curve

Concentration (pg/mL)	OD	Corrected OD
3000.00	2.153	2.072
1500.00	1.593	1.512
750.00	1.186	1.105
375.00	0.871	0.790
187.50	0.468	0.387
93.75	0.325	0.244
46.88	0.217	0.136
0.00	0.081	0.000

Sample Type

Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates, Other biological fluids

Limit Of Detection

3000 pg/mL

Precision

Matrices listed below were spiked with certain level of recombinant MAP4K4 and the recovery rates were calculated by comparing the measured value to the expected amount of MAP4K4 in samples.

Matrix	Recovery range	Average
serum(n=5)	80-97%	88%
EDTA Plasma(n=5)	78-92%	85%
Heparin Plasma(n=5)	90-105%	97%

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MAP4K4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MAP4K4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MAP4K4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MAP4K4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Manual
MSDS

UniProt ID	O95819

Research Area	Enzyme & Kinase;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15209077	ELK0209	48T	$412		Add to Cart
BHE15209077	ELK0209	96T	$588		Add to Cart

Human MAP4K4(Mitogen-activated Protein Kinase 4) ELISA Kit

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