Human Mannose-binding Protein C

Mfr.No.

E4304hu

Sensitivity

2.41ng/ml

Detection Range

5-1000ng/ml

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Collectin-1;Mannan-binding protein;Mannose-binding lectin;Mannose-binding protein C;MBL 2;MBL2;MBP1;MBP-C

Molecular Weight

26144

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4304hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	282.2	18.52	6.6
Intra-Assay	2	18	296.8	10.27	3.5
Intra-Assay	3	18	123.4	8.46	6.9

Background

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.

Protocol

These standard curves of E4304hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
640ng/ml	2.188	2.241	2.194
	2.295
320ng/ml	1.256	1.336	1.288
	1.416
160ng/ml	0.741	0.816	0.768
	0.89
80ng/ml	0.529	0.496	0.449
	0.463
40ng/ml	0.266	0.27	0.222
	0.274
0ng/ml	0.05	0.048	0
	0.045

Gene ID	4153
UniProt ID	P11226

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105682	E4304hu	48T	$320		Add to Cart
BHE12105682	E4304hu	96T	$458		Add to Cart

Human Mannose-binding Protein C - MBL2 ELISA Kit

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