Human Mannan-binding Lectin Serine Peptidase 2

Mfr.No.

E5633Hu

Sensitivity

7.47ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Mannan-binding lectin serine protease 2;Mannan-binding lectin serine protease 2 A chain;Mannan-binding lectin serine protease 2 B chain;Mannose-binding protein-associated serine protease 2;MASP 2;MASP2;MASP-2;MBL-associated serine protease 2

Molecular Weight

75702

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5633Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1287.3	59.63	4.6
Intra-Assay	2	18	871.6	55.55	6.4
Intra-Assay	3	18	489.2	23.91	4.9

Background

Serum protease that plays an important role in the activation of the complement system via mannose-binding lectin. After activation by auto-catalytic cleavage it cleaves C2 and C4, leading to their activation and to the formation of C3 convertase.

Protocol

These standard curves of E5633Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	1.721	1.732	1.681
	1.744
800ng/L	1.134	1.085	1.034
	1.036
400ng/L	0.553	0.493	0.441
	0.433
200ng/L	0.239	0.229	0.177
	0.219
100ng/L	0.149	0.114	0.062
	0.08
0ng/L	0.052	0.052	0
	0.051

Gene ID	10747
UniProt ID	O00187

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106995	E5633Hu	48T	$320		Add to Cart
BHE12106995	E5633Hu	96T	$458		Add to Cart

Human Mannan-binding Lectin Serine Peptidase 2 - MASP2 ELISA Kit

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