Human Manganese-dependent Adp-Ribose - CDP-alcohol Diphosphatase

Mfr.No.

E4248hu

Sensitivity

7.23ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of human Manganese-dependent ADP-ribose, CDP-alcohol Diphosphatase (also known as C17orf48) in serum, plasma, cell culture supeates, cell lysates, tissue homogenates.

Alternative Names

ADPRibase-Mn;C17orf48;C17orf48 ;CDP-choline phosphohydrolase;Manganese-dependent ADP-ribose/CDP-alcohol diphosphatase;MDS006;NBLA03831

Molecular Weight

39529

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supeatants, Plasma

Precision

We measured random samples of E4248hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	407.8	22.75	5.58
Intra-Assay	2	18	1106.22	33.75	3.05
Intra-Assay	3	18	280.33	19.32	6.89

Background

Hydrolyzes ADP-ribose, IDP-ribose, CDP-glycerol, CDP-choline and CDP-ethanolamine, but not other non-reducing ADP-sugars or CDP-glucose. May be involved in immune cell signaling as suggested by the second-messenger role of ADP-ribose, which activates TRPM2 as a mediator of oxidative/nitrosative stress (By similarity).

Protocol

These standard curves of E4248hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.317	2.254	2.217
	2.19
800ng/L	1.215	1.179	1.142
	1.142
400ng/L	0.775	0.797	0.76
	0.819
200ng/L	0.459	0.456	0.419
	0.454
100ng/L	0.187	0.22	0.184
	0.253
0ng/L	0.033	0.037	0
	0.04

Gene ID	56985
UniProt ID	Q3LIE5

Research Area	Signal Transduction

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105643	E4248hu	48T	$320		Add to Cart
BHE12105643	E4248hu	96T	$458		Add to Cart

Human Manganese-dependent Adp-Ribose - CDP-alcohol Diphosphatase - ADPRM ELISA Kit

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