Human MANF ELISA Kit PicoKine®

Mfr.No.

EK1845

Sensitivity

<15 pg/ml

Detection Range

93.6 pg/ml - 6,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	93.8	187.5	375	750	1500	3000	6000
O.D.	0.063	0.091	0.109	0.171	0.354	0.654	1.194	1.885

Description

Human MANF ELISA Kit PicoKine® (96 Tests). Quantitate Human MANF in cell culture supeatants, serum and plasma (heparin, EDTA). Sensitivity: 15 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; MANF; ARMET; ARP

Immunogen

Expression system for standard: NS0; Immunogen sequence: L25-L182

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human MANF

Subcellular Localization

Endoplasmic reticulum lumen, Secreted, Sarcoplasmic reticulum lumen

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	156	733	2725	166	748	2871
Standard deviation	9.67	47.65	125.35	8.47	55.35	195.23
CV (%)	6.2%	6.5%	4.6%	5.1%	7.4%	6.8%

Protein Function

Selectively promotes the survival of dopaminergic neurons of the ventral mid-brain. Modulates GABAergic transmission to the dopaminergic neurons of the substantia nigra. Enhances spontaneous, as well as evoked, GABAergic inhibitory postsynaptic currents i

Background

MANF is a 20kDa protein which belongs to the ARMET family. This gene is mapped to 3p21.2. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	156	148	167	169	160	8.51	5.3%
Sample 2	733	654	771	713	717	42.29	5.8%
Sample 3	2725	2678	2428	2740	2642	126.07	4.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	7873
Gene Name	MANF
UniProt ID	P55145

Applications	ELISA
Research Area	Amino Acids,Cancer,Cell Cycle,Cell Division,Neuroscience,Neurotransmitter

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001418	EK1845	96 wells/kit	$750		Add to Cart

Human MANF ELISA Kit PicoKine®

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