Human Macrophage Inflammatory Protein-1beta

Mfr.No.

E0087Hu

Description

This sandwich kit is for the accurate quantitative detection of human Macrophage Inflammatory Protein-1 Beta (also known as MIP-1B) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

ACT-2;C-C motif chemokine 4;CCL 4;CCL4;G-26 T-lymphocyte-secreted protein;HC21;LAG-1;Lymphocyte activation gene 1 protein;Macrophage inflammatory protein 1-beta;MIP-1-beta;MIP-1-beta(1-69;MIP-1-beta(3-69;PAT 744;Protein H400;SIS-gamma;Small-inducible cytokine A4;T-cell activation protein 2

Reactivity

Human

Detection Range

0.5-150pg/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

10212

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

0.22pg/ml

Background

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

Gene ID	3883726351
UniProt ID	P13236
Antigen	CCL4

Research Area

Immunology

Protocol

These standard curves of E0087Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
80pg/ml	2.356	2.39	2.337
	2.424
40pg/ml	1.541	1.562	1.51
	1.583
20pg/ml	0.935	0.879	0.826
	0.823
10pg/ml	0.43	0.442	0.39
	0.455
5pg/ml	0.252	0.264	0.212
	0.277
0pg/ml	0.043	0.053	0
	0.063

Precision

We measured random samples of E0087Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	50.9	1.4	2.7
Intra-Assay	2	18	8.7	0.43	5
Intra-Assay	3	18	29.6	1.75	5.9

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12101822	E0087Hu	96T	$458		Add to Cart
BHE12101822	E0087Hu	48T	$320		Add to Cart

Human Macrophage Inflammatory Protein-1beta - MIP-1B ELISA Kit

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