Human M-Cadherin-15 CDH15 ELISA Kit PicoKine®

Mfr.No.

EK2099

Sensitivity

<50 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Human

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.006	0.074	0.170	0.311	0.638	1.084	1.772	2.293

Description

Human M-Cadherin-15 CDH15 ELISA Kit PicoKine® (96 Tests). Quantitate Human CDH15 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Cadherin-15; Cadherin-14; Muscle cadherin; M-cadherin; CDH15; CDH14; CDH3

Immunogen

Expression system for standard: NS0; Immunogen sequence: L61-A606

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human Cadherin-15

Subcellular Localization

Cell membrane. Single-pass type I membrane protein.

Sample Type

Cell culture supernatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	255	1011	3863	264	1095	4861
Standard deviation	10.46	43.47	177.70	13.46	64.61	325.69
CV (%)	4.1%	4.3%	4.6%	5.1%	5.9%	6.7%

Protein Function

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. M-cadherin is part of the myogenic program and may provide a trigger for terminal muscle differentiation.

Background

Cadherin-15 is a protein that in humans is encoded by the CDH15 gene. It is mapped to 16q24.3. This gene is a member of the cadherin superfamily of genes, encoding calcium-dependent intercellular adhesion glycoproteins. Cadherins consist of an extracellular domain containing 5 cadherin domains, a transmembrane region, and a conserved cytoplasmic domain. Transcripts from this particular cadherin are expressed in myoblasts and upregulated in myotubule-forming cells. The protein is thought to be essential for the control of morphogenetic processes, specifically myogenesis, and may provide a trigger for terminal muscle cell differentiation.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	255	267	282	292	274	14.12	5.1%
Sample 2	1011	892	904	880	921	52.22	5.6%
Sample 3	3863	4003	3436	3659	3740	214.05	5.7%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	1013
Antigen	M-Cadherin-15
Gene Name	CDH15
UniProt ID	P55291
Tissue Specificity	Expressed in the brain and cerebellum.

Applications	ELISA

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001645	EK2099	96 wells/kit	$750		Add to Cart

Human M-Cadherin-15 CDH15 ELISA Kit PicoKine®

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