Human Lysosomal Pro-X Carboxypeptidase

Mfr.No.

E4257hu

Sensitivity

0.023ng/mL

Detection Range

0.5-8ng/mL

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Human Prolylcarboxypeptidase (also known as PRCP) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Angiotensinase C;Lysosomal carboxypeptidase C;Lysosomal Pro-X carboxypeptidase;PRCP;Proline carboxypeptidase;Prolylcarboxypeptidase

Molecular Weight

55800

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E4257hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	0.9	0.06	7
Intra-Assay	2	18	1.6	0.1	6.2
Intra-Assay	3	18	2.6	0.13	4.9

Background

Cleaves C-terminal amino acids linked to proline in Peptides such as angiotensin II, III and des-Arg9-bradykinin. This cleavage occurs at acidic pH, but enzymatic activity is retained with some substrates at neutral pH.

Protocol

These standard curves of E4257hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
8ng/mL	2.223	2.214	2.152
	2.204
4ng/mL	1.608	1.664	1.602
	1.72
2ng/mL	1.187	1.191	1.13
	1.195
1ng/mL	0.728	0.735	0.673
	0.742
0.5ng/mL	0.433	0.455	0.394
	0.477
0ng/mL	0.057	0.062	0
	0.066

Gene ID	5547
UniProt ID	P42785

Research Area	Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12105651	E4257hu	48T	$320		Add to Cart
BHE12105651	E4257hu	96T	$458		Add to Cart

Human Lysosomal Pro-X Carboxypeptidase - PRCP ELISA Kit

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