Human Lysosomal Acid Lipase Cholesteryl Ester Hydrolase

Mfr.No.

E5612Hu

Sensitivity

14.22ng/L

Detection Range

30-9000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Acid cholesteryl ester hydrolase;CESD;Cholesteryl esterase;LAL;LIPA;Lipase A;Lysosomal acid lipase/cholesteryl ester hydrolase;Sterol esterase

Molecular Weight

45419

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5612Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	2058.78	79.5	3.86
Intra-Assay	2	18	3748.75	85.21	2.27
Intra-Assay	3	18	1823.39	74.63	4.09

Background

Catalyzes the deacylation of triacylglyceryl and cholesteryl ester core lipids of endocytosed low density lipoproteins to generate free fatty acids and cholesterol.

Protocol

These standard curves of E5612Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
4800ng/L	2.08	2.093	2.048
	2.107
2400ng/L	0.899	0.989	0.944
	1.079
1200ng/L	0.527	0.48	0.435
	0.433
600ng/L	0.188	0.201	0.156
	0.214
300ng/L	0.089	0.091	0.046
	0.093
0ng/L	0.05	0.045	0
	0.039

Gene ID	3988
UniProt ID	P38571

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106974	E5612Hu	48T	$320		Add to Cart
BHE12106974	E5612Hu	96T	$458		Add to Cart

Human Lysosomal Acid Lipase Cholesteryl Ester Hydrolase - LIPA ELISA Kit

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