Human LYPD3 ELISA Kit PicoKine®

Mfr.No.

EK1837

Sensitivity

<0.1 ng/ml

Detection Range

0.312 ng/ml - 20 ng/ml

Reactivity

Human

Sample Volume

Concentration (ng/ml)	0	0.3	0.63	1.25	2.5	5	10	20
O.D.	0.001	0.201	0.350	0.599	0.929	1.351	1.725	2.196

Description

Human LYPD3 ELISA Kit PicoKine® (96 Tests). Quantitate Human LYPD3 in cell culture supeatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.1ng/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Ly6/PLAUR domain-containing protein 3; GPI-anchored metastasis-associated protein C4.4A homolog; Matrigel-induced gene C4 protein; MIG-C4; LYPD3; C4.4A

Immunogen

Expression system for standard: NS0; Immunogen sequence: L31-C326

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant human LYPD3

Subcellular Localization

Cell membrane, GPI-anchor

Sample Type

Cell culture supeatants, Serum, Plasma

Anticoagulant

heparin, EDTA or citrate

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	590	2019	10613	621	2312	11100
Standard deviation	25.96	102.97	583.72	40.99	171.09	843.60
CV (%)	4.4%	5.1%	5.5%	6.6%	7.4%	7.6%

Protein Function

Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.

Background

Ly6/PLAUR domain-containing protein 3 is a protein that in humans is encoded by the LYPD3 gene. This gene is mapped to 19q13.31.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	590	572	604	542	577	23.17	4.0%
Sample 2	2019	2207	2161	2108	2123	69.89	3.2%
Sample 3	10613	9014	10567	8909	9775	815.25	8.3%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	27076
Gene Name	LYPD3
UniProt ID	O95274
Tissue Specificity	Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TC

Applications	ELISA
Research Area	Cancer,Tumor Biomarkers

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21001413	EK1837	96 wells/kit	$750		Add to Cart

Human LYPD3 ELISA Kit PicoKine®

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