Human Lupus La Protein - SSB ELISA Kit

Mfr.No.

E5603Hu

Sensitivity

7.14ng/L

Detection Range

15-3000ng/L

Reactivity

Human

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

La;La autoantigen;La ribonucleoprotein;La/SSB;LARP3;Lupus La protein;Sjoegren syndrome type B antigen;SSB;SS-B;

Molecular Weight

46837

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sample Type

Serum, Cell culture supernatants, Plasma

Precision

We measured random samples of E5603Hu within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	407.48	19.56	4.8
Intra-Assay	2	18	935.16	32.2	3.44
Intra-Assay	3	18	196.13	12.12	6.18

Background

Binds to the 3' poly(U) terminus of nascent RNA polymerase III transcripts, protecting them from exonuclease digestion and facilitating their folding and maturation (PubMed:3192525, PubMed:2470590). In case of Coxsackievirus B3 infection, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation (PubMed:12384597).

Protocol

These standard curves of E5603Hu are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
1600ng/L	2.087	2.101	2.056
	2.114
800ng/L	1.163	1.267	1.223
	1.371
400ng/L	0.732	0.759	0.715
	0.787
200ng/L	0.441	0.414	0.37
	0.388
100ng/L	0.17	0.179	0.134
	0.188
0ng/L	0.029	0.044	0
	0.059

Gene ID	6741
UniProt ID	P05455

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12106965	E5603Hu	48T	$320		Add to Cart
BHE12106965	E5603Hu	96T	$458		Add to Cart

Human Lupus La Protein - SSB ELISA Kit

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